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S4047 Sigma

Anti-S6 Kinase antibody produced in rabbit

whole antiserum

Synonym: Anti-p70S6k

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Primary Antibodies,
species reactivity   rat, mouse, human
application(s)   microarray: suitable
  western blot: 1:10,000 using mouse NIH 3T3 fibroblast cell extract
clone   polyclonal
antibody form   whole antiserum
mol wt   antigen mol wt 70 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... RPS6KB1(6198)
mouse ... Rps6kb1(72508)
rat ... Rps6kb1(83840)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 6-23 of human S6 kinase.

General description

S6 kinases are Ser/Thr protein kinases that are capable of phosphorylating the 40S ribosomal protein S6 in response to mitogen stimulation. S6 kinase (p70s6k) is activated by an upstream kinase, mTOR in response to nutrients and growth factors. S6 kinase (p70s6k) is a critical regulatory component and mTOR along with the eukaryotic initiation factor 4E (eIF4E)–binding protein 1 (4E-BP1) and S6 kinase (p70s6k) leads to increased ribosomal biogenesis and hence translation. As a critical component and has the ability to modify the cellular responses to availability of nutrients, and growth factors like insulin and IGF-1 when mTOR is chronically active. In presence of excess and chronic insulin, or high fat diets, S6 kinase (p70s6k) regulates by a negative feedback loop and constitutively shuts down the responsiveness of cells to insulin. Thus mTOR through the S6 kinase (p70s6k) plays a pivotal role in the development of insulin resistance and type 2 diabetes. mTOR signaling via p70S6 kinase also drives cell growth and proliferation, cancer and aging.
Anti-S6 Kinase reacts specifically with S6 kinase (70 kDa) in human, rat and mouse.

Specificity

The sequence of the immunizing peptide is identical in rat and mouse S6K.

Application

Anti- S6 kinase antibody may be used for detection by immunoblotting at a working dilution of 1:10,000 using mouse NIH 3T3 fibroblast cell extract. Detection by immunoblotting was also done in primary cultures of mouse glial cells and in LnCAP, prostate cancer cell line.

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