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S5059 Sigma

SigmaSpin Sequencing Reaction Clean-Up

post-reaction clean-up columns

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Description

Application

Ideal for removing dye-terminator nucleotides and primers from sequencing reactions and radiolabeled nucleotides, primers, and fluorescent dyes from nucleic acid probe labeling reactions.

SigmaSpin® Sequencing Reaction Clean-Up has been used in the synthesis of antisense RNA probe.

Other Notes

SigmaSpin Post-Reaction Clean-Up Columns are ideal for lower throughput applications, such as clean-up of probe labeling reactions or small numbers of sequencing reactions. These columns can accept sample volumes up to 100 μL. Each column comes with a collection tube to collect the DNA during centrifugation.

SigmaSpin Post-Reaction Clean-Up columns provide a fast, simple, and highly efficient method for removing unincorporated dyes, excess salts, and other interfering reaction components. Each column is packed with a pre-hydrated size-exclusion resin, equilibrated with molecular biology grade water, and supplied in our unique column design. The column also includes a snap-cap bottom seal and an o-ring containing screw-cap to ensure that the resin remains hydrated. SigmaSpin has been tested in high-throughput genome centers and core facilities with ABI PRISM® 3700, 3100, 310 and 377. Each well can accept sample volumes up to 20 μL.

For additional information, please see www.sigma-aldrich.com/sequencing.

Legal Information

ABI PRISM is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

SabreLite is a registered trademark of Pelican Products, Inc.

SigmaSpin is a trademark of Sigma-Aldrich Co. LLC

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Microcon Centrifugal Filters

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