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S5174 Sigma

SPARC from murine parietal yolk sac (PYS-2) cells

~80% (SDS-PAGE), lyophilized powder

Synonym: BM-40, Osteonectin, Secreted protein acidic and rich in cysteine



Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes, Cell Signaling and Neuroscience,
assay   ~80% (SDS-PAGE)
form   lyophilized powder
surface coverage   4‑40 μg/cm2
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Sparc(20692)



SPARC, or Secreted Protein Acidic and Rich in Cysteine, is a 283-287 AA Ca2+ binding to glycoprotein with an apparent molecular weight of 40-43 kDa.


SPARC has many useful properties including inhibiting cell spreading and diminishing focal contacts in vitro, abrogating the effects of PDGF and beta-FGF, binding directly to several components of the ECM, and undergoes proteolysis that exposes and releases regions in the protein that correlates with biological activities.

Biochem/physiol Actions

SPARC is expressed in many tissues including bone, skin, skeletal muscle, reproductive tissues, and embryonic connective tissue. It is expressed in high levels in tissues that are undergoing morphogenesis, remodeling and repair.


The lyophilized powder should be stored frozen at -20°C. The reconstituted product should be stored frozen at -70°C.

Preparation Note

This product is supplied as a lyophilized powder from phosphate buffered saline at 25 μg protein/vial. It should be reconstituted aseptically with sterile water, producing a solution of 0.1 to 0.3 mg protein per mL. SPARC is initially purified from mouse parietal yolk sac cells under conditions that preserve biological activity.

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


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Keywords: Angiogenesis, Cancer, Eliminations, Gene expression, Growth factors, Ligands, Metabolic Pathways, Phosphorylations

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