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S5174 Sigma

SPARC from murine parietal yolk sac (PYS-2) cells

~80% (SDS-PAGE), lyophilized powder

Synonym: BM-40, Osteonectin, Secreted protein acidic and rich in cysteine

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Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes, Cell Signaling and Neuroscience,
assay   ~80% (SDS-PAGE)
form   lyophilized powder
surface coverage   4‑40 μg/cm2
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Sparc(20692)

Description

Components

SPARC, or Secreted Protein Acidic and Rich in Cysteine, is a 283-287 AA Ca2+ binding to glycoprotein with an apparent molecular weight of 40-43 kDa.

Application

SPARC has many useful properties including inhibiting cell spreading and diminishing focal contacts in vitro, abrogating the effects of PDGF and beta-FGF, binding directly to several components of the ECM, and undergoes proteolysis that exposes and releases regions in the protein that correlates with biological activities.

Biochem/physiol Actions

SPARC is expressed in many tissues including bone, skin, skeletal muscle, reproductive tissues, and embryonic connective tissue. It is expressed in high levels in tissues that are undergoing morphogenesis, remodeling and repair.

Caution

The lyophilized powder should be stored frozen at -20°C. The reconstituted product should be stored frozen at -70°C.

Preparation Note

This product is supplied as a lyophilized powder from phosphate buffered saline at 25 μg protein/vial. It should be reconstituted aseptically with sterile water, producing a solution of 0.1 to 0.3 mg protein per mL. SPARC is initially purified from mouse parietal yolk sac cells under conditions that preserve biological activity.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

The Cancer Stem Cell Hypothesis

Traditionally, cancer has been viewed as a disease in which environmental or endogenous events induce mutations to critical oncogenes and tumor suppressor genes within a normal cell. The clinical man...
Keywords: Apoptosis, Asymmetric synthesis, Cancer, Catalysis, Cell culture, Cell division, Cell proliferation, Cell signaling, Clinical, Degradations, Environmental, Gastrointestinal, Gene expression, Ligands, Phosphorylations, Poisons, Transcription, Transfection, transformation

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Peer-Reviewed Papers
15

References

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