• USA Home
  • S5188 - Anti-SGK antibody produced in rabbit

EMAIL THIS PAGE TO A FRIEND
S5188 Sigma

Anti-SGK antibody produced in rabbit

buffered aqueous solution, IgG fraction of antiserum

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Primary Antibodies,
species reactivity   human
application(s)   indirect immunofluorescence: 1:2,000 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated human epidermal carcinoma A431cell line
  microarray: suitable
  western blot: 1:2,000 using whole cell extract of the human epidermal carcinoma A431 cell line
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 50 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... SGK1(6446)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the C-terminus of human SGK (amino acids 412-431).

General description

SGK (serum and glucocorticoid-regulated protein kinase) is a 50 kDa member of Ser/Thr kinase family. It is regulated at the transcription level by stimuli including glucocorticoids, serum, follicle stimulating hormone as well as changes in cell volume. Sequence of sgk is well conserved across species, the human and rat SGK amino acid sequences are 96-98% identical. SGK plays an important role in signal transduction activated by mineral corticoids. SGK is transcriptionally regulated by the glucocorticoid response element present in sgk promoter. SGK levels are strongly affected by osmotic changes that in changes in the cell volume. SGK is strongly and rapidly stimulated in kidney cells in response to aldosterone, and stimulates epithelial Na+ channels, suggesting a central role of SGK in the regulation of sodium transport and homeostasis. SGK transcription is markedly increased in diabetic nephropathy, in response to excessive extracellular glucose concentrations. The localization of SGK in the cell is regulated by hormones or serum. For cell cycle progression, the shuttling of SGK between nucleus and cytoplasm is suggested as a requirement.
Anti-SGK recognizes human SGK (50 kDa, appearing as a doublet).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Application

Anti-SGK antibody can be used at a minimum working dilution of 1:2,000 for detection by immunoblotting in whole extract of the human epidermal carcinoma A431 cell line. Indirect can be performed on human epidermal carcinoma A431 cell line at a working dilution of 1:2,000. The antibody is suitable for protein microarray.

Price and Availability


Antibody Explorer

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?