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  • S5313 - Anti-Sir2 (AS-16) antibody produced in rabbit

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S5313 Sigma

Anti-Sir2 (AS-16) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers and Receptors, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology,
species reactivity   mouse
application(s)   indirect immunofluorescence: 1:50 using mouse NIH3T3 cells
  microarray: suitable
  western blot: 1:2,000 using mouse NIH/3T3 cells
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~110 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Sirt1(93759)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to mouse Sir2 sequence (amino acids 722-737 with N-terminal added lysine), conjugated to KLH. This sequence is 62% homologous to the corresponding human sequence.

General description

Sir2, one of the silent information regulator genes, encodes a protein that promotes a compact chromatin structure, thereby preventing or silencing gene transcription at selected loci. Sir2 belongs to a family of proteins that is found in organisms ranging from bacteria to complex eukaryotes. Members of this family contain a 250 amino acid core domain that shares about 25-60% sequence identity.
Silencing occurs as a series of events initiated by formation of Sir complexes (Sir2, Sir3, Sir4). The complexes are recruited to their chromosome targets via interactions with DNA-binding proteins, followed by deacetylation of histones H3 and H4. A final step required for telomeric silencing is binding of the complex to the deacetylated histones and recruitment of the telosome to the nuclear periphery.
Sir2 protein is an NAD-dependent histone deacetylase, an enzyme that removes acetyl groups from lysine residues of histone proteins and possibly other substrates. Sir2 transfers acetyl groups from its protein substrates to ADP-ribose and synthesizes o-acetylADP-ribose. It appears that Sir2 NAD requirement makes this protein an important player in the pathway that leads to increased life span of several species through calorie restriction. The maintenance or silencing of chromatin may be at the center of processes leading to aging of cells and development of cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

Anti-Sir2 polyclonal antibody specifically recognizes mouse Sir2 (AS-16). Staining of the Sir2 band is specifically inhibited by the immunizing peptide.

Application

Anti-Sir2 (AS-16) antibody may be used to detect Sir2 by immunoblotting (approximately 110 kDa) and immunofluorescence applications.

Price and Availability


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