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S5393 Sigma

Serum minus IgA/IgM/IgG human

lyophilized powder

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Properties

Related Categories Antibodies, Human Serum Controls, Sera and Immunoglobulins, Supplementary Products
form   lyophilized powder
contains   15 mM sodium azide
packaging   vial of 0.5 mL
conjugate   unconjugated
storage temp.   2-8°C

Description

Quantity

Each vial contains at least 25 mg protein

Reconstitution

Reconstitute with 0.5 mL deionized water.

Application

Serum minus IgA/IgM/IgG human can be used as a control serum in different immunoassays like immunodiffusion, ELISA or immunoelectrophoresis. It can also be used in 25-plex PnPS assay.

General description

Serum is the liquid component of blood that includes proteins, immunoglobulin and electrolytes. The product can be used for selective removal of contaminating antibodies when immobilized on a solid matrix. This product can also be used in anti-protective antigen (anti-PA) IgG measurements using PA coupled microspheres. Human serum deficient of IgG, IgA, and IgM can react specifically with anti-human whole serum but does not show reactivity with anti-human IgG, IgA, or IgM.

Price and Availability


Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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