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S5772 Sigma

Sephadex® G-25

BioReagent, for molecular biology, DNA grade, Superfine

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Properties

Related Categories DNA & RNA Purification, Molecular Biology, Reagents More...
grade   DNA grade
  for molecular biology
product line   BioReagent
form   beads
bead diameter   20-50 μm
pore size   exclusion limit (10 bp dsDNA; (rA)10)
pH-range   2-13
foreign activity   DNase, RNase, none detected
storage temp.   room temp

Description

Features and Benefits

• Excellent recovery
• Minimal sample dilution
• Quick and easy to use

General description

Sephadex® G-25, Superfine is an often-cited gel filtration medium for desalting and buffer exchange. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Superfine” indicates the smallest particle size available.

Packaging

10, 25, 100 g in poly bottle

Application

Sephadex® G-25 is suitable for buffer exchange and desalting oligonucleotides for use in sequencing, PCR, and labeling applications. It was used to isolate two different peptides of Pronase E extracted from Alaska Pollack skin.

Legal Information

Sephadex is a registered trademark of GE Healthcare

Price and Availability


Microcon Centrifugal Filters

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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