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S5817 Sigma

SYPRO® Red Protein Gel Stain

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Description

Caution

Store desiccated and protect from light.

General description

SYPRO Red is similar to SYPRO Orange although it gives slightly lower background fluorescence and is not quite as bright as SYPRO Orange. For those using a laser-excited gel scanner, a He-Ne or Nd:YAG laser-based instrument is recommended for SYPRO Red. The dye is efficiently excited by UV or broad band illumination. It is not suitable for staining proteins on blots or in IEF gels and shows reduced sensitivity when staining proteins on 2-D gels.
SYPRO Red is:
• Highly sensitive. The stain can detect 1 to 2 ng of protein per mini gel band, making it more sensitive than Coomassie Brilliant Blue or silver staining.
• Rapid. Staining is complete in less than one hour.
• Simple. After electrophoresis, the gel is stained, rinsed and photographed; no fixation or destaining steps are required.
• Compatible with standard laboratory equipment. Stained proteins can be visualized with a standard 300 nm UV transilluminator or laser scanner.
• Cost-effective. Staining with SYPRO Red is less expensive than silver staining and requires less time.
• Low protein-to-protein variability. The dye interacts with the SDS coat around the protein, giving more consistent staining between different types of proteins compared to Coomassie or silver staining.
• Selective for proteins. SYPRO Red detects proteins as small as 6.5 kDa and does not stain nucleic acids or lipopolysaccharides. It does stain glycosylated proteins..
• Broad linear range of detection. The fluorescence intensity of the stained bands is linear with protein quantity over three orders of magnitude (a much broader range than either Coomassie or silver staining).

Legal Information

SYPRO is a registered trademark of Life Technologies

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NA 1993 / PGIII
WGK Germany 
3
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C

Documents

Certificate of Analysis

Protocols & Articles

Articles

Electrophoresis - Protein Staining Reagents

To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, als...
Keywords: PAGE, Proteomics

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