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S5897 Sigma

Sephadex® G-50

BioReagent, for molecular biology, DNA grade, fine

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Properties

Related Categories DNA & RNA Purification, Molecular Biology, Reagents More...
grade   DNA grade
  for molecular biology
product line   BioReagent
form   beads
swelling   1 g swells to 9-11 mL
bead diameter   20-80 μm
pore size   exclusion limit (20 bp dsDNA (rA)20)
pH   2 - 10
foreign activity   DNase, RNase, none detected
storage temp.   room temp

Description

Features and Benefits

• Excellent recovery
• Minimal sample dilution
• Quick and easy to use

General description

Sephadex® G-50, Fine is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Fine” indicates a small particle size known for excellent efficiency and short diffusion distances.

Packaging

25, 100 g in poly bottle

Application

Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing to improve the sequencing quality.

Legal Information

Sephadex is a registered trademark of GE Healthcare

Price and Availability


Microcon Centrifugal Filters

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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