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S6147 Sigma

Sephadex® G-100

BioReagent, for molecular biology, DNA grade, medium

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Properties

Related Categories DNA & RNA Purification, Molecular Biology, Reagents More...
grade   DNA grade
  for molecular biology
product line   BioReagent
form   beads
swelling   1 g swells to 9-11 mL
bead diameter   40-120 μm
pore size   exclusion limit (25 bp dsDNA; (rA)27)
pH   2 - 10
foreign activity   DNase, RNase, none detected
storage temp.   room temp

Description

Features and Benefits

• Excellent recovery
• Minimal sample dilution
• Quick and easy to use

General description

Sephadex® G-100, Medium is an often-cited gel filtration medium for desalting and buffer exchange of very large moleculaes. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Packaging

25, 100 g in poly bottle

Application

Sephadex® G-100 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify the enzyme polyphenoloxidases isolated from banana by gel filteration.

Legal Information

Sephadex is a registered trademark of GE Healthcare

Price and Availability


Microcon Centrifugal Filters

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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