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S6402 Sigma

Streptavidin−Cy3 from Streptomyces avidinii

buffered aqueous solution

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Properties

Related Categories Antibodies, Biotin/Avidin, Molecular Biology, Plant Biotechnology, Plant Proteomics,
form   buffered aqueous solution
extent of labeling   3-9 mol fluorochrome per mol protein
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
conjugate   CY3 conjugate
shipped in   wet ice
storage temp.   2-8°C

Description

Packaging

1 mL in glass bottle

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

Application

Streptavidin-Cy3 may be used in conjunction with biotinylated antibodies in immunohistochemistry and immunocytochemistry. Cy3 is excited maximally at 554 nm, emits at 568-574 nm, and can be used with the traditional rhodamine filter arrangements due to the spectral overlap. Cy3 has a molar extinction coefficient of 1.3 × 105 M.

General description

Streptavidin is a homotetramer protein. One molecule of streptavidin binds four molecules of biotin by a non-covalent interaction that is essentially irreversible.

Legal Information

Cy3 is a trademark of GE Healthcare

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

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