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S6436 Sigma

Anti-phospho-S6-Kinase (pThr421/pSer424) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-phospho-p70S6K (pThr421/pSer424)

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins at Phosphothreonine (pThr),
species reactivity   rat, human, mouse
application(s)   immunoprecipitation: 1:250
  western blot (chemiluminescent): 1:1,000
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... RPS6KB1(6198)
mouse ... Rps6kb1(72508)
rat ... Rps6kb1(83840)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic double phospho-Thr421/Ser424 peptide corresponding to residues around Thr421/Ser424 of human p70S6K.

General description

S6 kinase (p70s6k) is activated by an upstream kinase, mTOR in response to nutrients and growth factors. S6 kinase (p70s6k) is a critical regulatory component and mTOR along with the eukaryotic initiation factor 4E (eIF4E)–binding protein 1 (4E-BP1) and S6 kinase (p70s6k) leads to increased ribosomal biogenesis and hence translation. As a critical component and has the ability to modify the cellular responses to availability of nutrients, and growth factors like insulin and IGF-1 when mTOR is chronically active. In presence of excess and chronic insulin, or high fat diets, S6 kinase (p70s6k) regulates by a negative feedback loop and constitutively shuts down the responsiveness of cells to insulin. Thus, mTOR through the S6 kinase (p70s6k) plays a pivotal role in the development of insulin resistance and type 2 diabetes. mTOR signaling via p70S6 kinase also drives cell growth and proliferation, cancer and aging.
Anti-phospho-S6-kinase (p70S6K) (phosphothreonine 421/phosphoserine424) detects p70 S6 kinase and p85 S6 kinase only when activated by phosphorylation at Thr421/Ser424 and does not cross-react with other phosphoylated protein kinases.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol

Specificity

Does not react with non-phosphorylated S6 kinase (p70S6K) or other phosphorylated protein kinases.

Application

Anti-phospho-S6-kinase (p70S6K) may be used for immunoblotting at a working dilution of 1:1000 in serum-treated NIH-3T3 cells. For immunoprecipitation, the recommended dilultion is 1:250.

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