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  • S6558 - Anti-Ste20/PAK, Subdomain VI (From Yeast) antibody produced in rabbit

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S6558 Sigma

Anti-Ste20/PAK, Subdomain VI (From Yeast) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, ST-ST
biological source   rabbit
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous glycerol solution
species reactivity   yeast, rat, rabbit
application(s)   immunoprecipitation: 4 μg using Ste20 isoforms from 0.5–1 mg of a rabbit brain cytosol.
  western blot (chemiluminescent): 2 μg/mL using a whole brain extract from a rat or rabbit
conjugate   unconjugated
shipped in   wet ice
storage temp.   −20°C

Description

Immunogen

synthetic peptide corresponding to the subdomain VI of S. cerevisiae Ste20 (amino acids 738-752) conjugated to KLH.

Specificity

By immunoblotting, the antibody recognizes recombinant yeast Ste20 protein, expressed in Escherichia. coli. It also reacts with a 65 kDa protein in rabbit and rat brain that corresponds to PAK. PAK is the Ste20-like kinase that interacts with the small G proteins Rac and Cdc42. The antibody cross-reacts with other proteins in rat tissue extracts that may be homologs of PAK.

Physical form

Solution in 0.07 M Tris-glycine, pH 7.0, containing 30% glycerol

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Safety & Documentation

Safety Information

WGK Germany 
1
Protocols & Articles

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