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S7025 Sigma

50 bp DNA Step Ladder

for DNA electrophoresis

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Properties

Related Categories Core Bioreagents, DNA Ladder Markers, Life Science Reagents for DNA/RNA Electrophoresis, Markers and Ladders, Molecular Biology,
form   liquid
usage   100 uses
suitability   suitable for electrophoresis (DNA)
storage temp.   −20°C

Description

Components

Sigma’s 50 bp DNA Step Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 10 mM NaCl, and 1 mM EDTA.

General description

Sigma’s 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. For NA electrophoresis, 10 μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for Northern and Southern blotting.

Other Notes

Contains blunt-ended fragments of DNA, 50-500 bp by 50, 600-900 bp by 100, and 1000-3000 bp by 1000 (17 bands).

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Application

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Introduction to Markers and Ladders

Sigma-Aldrich offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments ...
Keywords: Digestions, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Markers and Ladders Selection Guide

DNA and RNA size markers contain a mixture of DNA (or RNA) fragments of known length, making them suitable for estimating the fragment length of concurrently run samples. They stain well with ethidiu...
Keywords: AGE, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Peer-Reviewed Papers
15

References

Related Products

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D5042 123 bp DNA Ladder, for DNA electrophoresis
G2526 Gel Loading Buffer, for NA electrophoresis
D3937 DirectLoad 1 kb DNA Ladder, ready-to-use marker for DNA electrophoresis
D0428 1 kb DNA Ladder, for DNA electrophoresis

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