• USA Home
  • S7901 - Stem Cell Factor human

EMAIL THIS PAGE TO A FRIEND
S7901 Sigma

Stem Cell Factor human

SCF, recombinant, expressed in E. coli, powder, suitable for cell culture

Synonym: SCF, c-Kit ligand

Purchase

Description

Analysis Note

The proliferative activity is tested in culture using TF-1 cells or by the dose dependent proliferation of human MO-7e cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in PBS containing 500 μg BSA /μg cytokine.

Biochem/physiol Actions

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Hematopoietic Cytokines

Hematopoietic cytokines are large family of extracellular ligands that stimulate hematopoietic cells to differentiate into eight principle types of blood cells. Numerous cytokines are involved in the...
Keywords: Cell proliferation, Clinical, Growth factors, Ligands

Protocols

Induced Pluripotent Stem Cell Reprogramming Protocols

Introduction Methods     Reprogramming of Human Fibroblasts using Non-Integrating Self-Replicating RNA Vectors     Reprogramming of Peripheral Blood Mononuclear Cells (PBMCs) using STEMCCA Lentiviral...
Keywords: Apoptosis, Cell culture, Culture media, Gene expression, Growth factors, Immunocytochemistry, Polymerase chain reaction, Respiratory, Transduction, Transfection

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?