Stem Cell Factor human recombinant, expressed in E. coli, powder, suitable for cell culture

Properties

Related Categories	Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Core Bioreagents, Cytokines and Growth Factors, Cytokines, Growth Factors and Chemokines for Stem Cell Differentiation, Cytokines, Growth Factors and Hormones, Cytokines, Growth Factors, Chemokines for Stem Cell Biology, Cytokines, Growth Factors, and Chemokines for Stem Cell Expansion, General Reagents, General Stem Cell Biology, Growth Factors, Growth Factors and Cytokines, Hematopoietic Cytokines, Hematopoietic Stem Cell Biology, Hematopoietic Stem Cell Cytokine and Growth Factors, Life Science Reagents for RNAi, Reagents and Supplements, Research Essentials, Stem Cell Biology, Stem Cell Differentiation, Stem Cell Expansion, Stem Cell Factor (SCF) More...
recombinant	expressed in E. coli
assay	>97% (SDS-PAGE)
form	powder
potency	2.5-10 ng/mL ED₅₀/EC₅₀
mol wt	protein mol wt 18.5 kDa
impurities	endotoxin, tested
suitability	suitable for cell culture
storage temp.	−20°C

Show More (9)

Description

Analysis Note

The proliferative activity is tested in culture using TF-1 cells or by the dose dependent proliferation of human MO-7e cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in PBS containg 50 μg BSA /μg cytokine.

Biochem/physiol Actions

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.