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S8409 Sigma

Superoxide Dismutase from bovine liver

ammonium sulfate suspension, 2,000-6,000 units/mg protein (biuret)

Synonym: SOD, Superoxide: superoxide oxidoreductase

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Properties

Related Categories 1.15.x.x Acting on superoxide radicals, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates More...
form   ammonium sulfate suspension
storage temp.   2-8°C
Gene Information   cow ... SOD1(281495)

Description

Analysis Note

For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).

Biochem/physiol Actions

Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

General description

Superoxide dismutases are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals and micro-organisms. They provide protection against damaging reactions with the superoxide radical anion (O2-) by catalyzing its disproportionation into oxygen and hydrogen peroxide.

Packaging

3000, 15000 units in glass bottle

Unit Definition

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.

Physical form

Suspension in 3.8 M (NH4)2SO4, pH 7.0

Application

Superoxide dismutase from bovine liver has been used in a study to assess the inactivation of endothelial derived relaxing factor by oxidized lipoproteins. Superoxide dismutase from bovine liver has also been used in a study to investigate the role of hydrogen peroxide in the cytotoxicity of the xanthine/xanthine oxidase system.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Ammonium Sulfate Suspensions - Technical Note

Note that for an ammonium sulfate suspension, most of the enzyme will be in solid form. It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution.
Keywords: Enzyme activity

Protocols

Enzymatic Assay of Superoxide Dismutase

This procedure applies to all products that have a specification for superoxide dismutase activity by enzymatic determination.
Keywords: Catalysis, Reductions

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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