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S8830 Sigma

SIGMAFAST Protease Inhibitor Cocktail Tablets, EDTA-Free

for use in purification of Histidine-tagged proteins

Synonym: Protease Inhibitor Cocktail

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon
Leupeptin
Aprotinin

Application

Optimized and tested for use with HIS-Select media.

General description

Tableted formulation containing water-soluble protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic and metalloproteases. Contains 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF), Bestatin hydrochloride, Leupeptin, E-64, Aprotinin, Pepstatin A, and Phosphoramidon disodium salt.

Quantity

One tablet makes 100 mL of cocktail. One tablet is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.

Specificity

Inhibits serine, cysteine, aspartic, and metalloproteases.

Physical form

Tablet that is water soluble

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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Safety & Documentation

Safety Information

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Protease Inhibitor Cocktails References

The Sigma-Aldrich portfolio of protease inhibitor cocktails shows an excellent track record in the peer-reviewed scientific literature, as witnessed by the many publications that cite our protease in...
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Peer-Reviewed Papers
15

References

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