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S8830 Sigma

SIGMAFAST Protease Inhibitor Cocktail Tablets, EDTA-Free

for use in purification of Histidine-tagged proteins

Synonym: Protease Inhibitor Cocktail

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon
Leupeptin
Aprotinin

Application

Optimized and tested for use with HIS-Select media.

General description

Tableted formulation containing water-soluble protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic and metalloproteases. Contains 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF), Bestatin hydrochloride, Leupeptin, E-64, Aprotinin, Pepstatin A, and Phosphoramidon disodium salt.

Quantity

One tablet makes 100 mL of cocktail. One tablet is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.

Specificity

Inhibits serine, cysteine, aspartic, and metalloproteases.

Physical form

Tablet that is water soluble

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Price and Availability


Pharma QC

Ultrafree Centrifugal Filter Units

Biomedical Applications
Safety & Documentation

Safety Information

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the difference between Protease Inhibitors P8849 and S8830?
P8849 is a DMSO solution and S8830 is a water soluble tablet. P8849 formulation is considered proprietary but does contain AEBSF, Pepstatin A, Bestatin, E-64, and Phosphoramidon. The formulation of S8830 also contains Aprotinin and Pepstatin A and the exact formulation is given on the Product Information Sheet (http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/5/s8830dat.Par.0001.File.tmp/s8830dat.pdf).
Can Product No. P8849, Protease Inhibitor Cocktail, be used with with HIS-Select™ products or any other immobilized metal affinity chromatography (IMAC) products?
This product does not contain EDTA and can be used with sample processing by immobilized metal affinity chromatography (IMAC) such as used for the purification of histidine-tagged proteins..
How should I dissolve Product No. S8830, SIGMAFAST™ Protease Inhibitor?
You can dissolve the product in 100 ml of water to make a 1X solution. You can make a 10X stock solution using 10 ml of water and then dilute 1:10 for use.  Concentrations greater than 1X may appear slightly hazy. This will not affect the performance of the protease inhibitors.This and other useful information can be found on the product data sheet: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/5/s8830dat.Par.0001.File.tmp/s8830dat.pdf
Are there publications that cite use of Product S8830, SIGMAFAST™ Protease Inhibitor Cocktail Tablets?
Yes. Several examples of published papers that cite the use of Product S8830 include these: 1.  Smith, E.R., et al., Instability of fibroblast growth factor-23 (FGF-23): Implications for clinical studies.  Clinica Chimica Acta, 412(11-12), 1008-1011 (2011).  Use was with blood at 20 µL cocktail per 1 mL of blood. (Direct link to paper:  http://www.sciencedirect.com/science/article/pii/S0009898111001008) 2.  Reber, S., "Isolation of Centrosomes from Cultured Cells" (Chapter 8), from Microtubule Dynamics: Methods in Molecular Biology (Springer Protocols), vol. 777, pp. 107-116 (2011). (Direct link:  http://www.springerlink.com/content/m34122064p131p61/#section=927892&page=1&locus=0)
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Protocols & Articles

Articles

Protease Inhibitor Cocktails References

The Sigma-Aldrich portfolio of protease inhibitor cocktails shows an excellent track record in the peer-reviewed scientific literature, as witnessed by the many publications that cite our protease in...
Keywords: AGE, Adhesion, Aerobic, Affinity chromatography, Alzheimer Disease, Amplification, Angiogenesis, Anti-inflammatory agents, Antibiotics, Antimicrobials, Antitumor agents, Antivirals, Apoptosis, Atomic absorption spectroscopy, Autophagy, Biochemistry, Biological processes, Buffers, Cancer, Cardiovascular, Catalysis, Cell biology, Cell disruption, Cell proliferation, Cell signaling, Cellular processes, Central Nervous System, Chromatin immunoprecipitation, Chromatography, Clinical, Cloning, Cytotoxins, DNA replication, Degradations, Detergents, Diabetes, Diagnostic, Diseases, Dopamine agents, Drug discovery, Eliminations, Environmental, Enzyme-linked immunosorbent assay, Gas chromatography, Gastrointestinal, Gene expression, Genetic, Genetics, Gluconeogenesis, Glycosylations, Growth factors, Hormones, Hydration reaction, Hydroxylations, Immobilization, Immunology, Immunoprecipitation, Inflammation, Ligands, Lipid Metabolism, Lipid peroxidation, Mass spectrometry, Medicinal chemistry, Metabolism, Metabolites, Methylations, Microarray Analysis, Microbiology, Microscopy, Molecular biology, Morphogenesis, Neurology, Neuroscience, Neurotransmission, Neurotransmitters, Normal-phase chromatography, Nutrition, Obesity, Oncology, PAGE, Parkinson Disease, Peroxidations, Pharmaceutical, Phosphorylations, Photosynthesis, Physiological Processes, Polymerase chain reaction, Polymorphisms, Proteomics, Purification, Recombination, Reductions, Reproduction, Respiratory, Reversible addition-fragmentation chain transfer polymerizations, Sample preparations, Scanning electron microscopy, Schizophrenia, Separation, Sequencing, Sonication, Transcription, Transduction, Veterinary, Vitamins, Western blot, transformation

Peer-Reviewed Papers
15

References

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