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S9008 Sigma

Anti-Staphylococcal Enterotoxin B antibody produced in rabbit

fractionated antiserum, lyophilized powder

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Properties

Related Categories Alphabetical Index, Antibodies, Miscellaneous, Primary Antibodies, ST-ST More...
biological source   rabbit
antibody form   fractionated antiserum
clone   polyclonal
form   lyophilized powder
species reactivity   Staphylococcus aureus
packaging   vial of 2 mL lyophilized antiserum
application(s)   indirect ELISA: 1:150,000-1:250,000
conjugate   unconjugated
storage temp.   2-8°C

Description

Immunogen

Staphylococcal enterotoxin B from Staphylococcus aureus.

Reconstitution

Reconstitute with 2 ml deionized water.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2.

Application

Anti-Staphylococcal Enterotoxin B antibody produced in rabbit was used in immunohistochemical staining of SEB-injected fore footpad sections of BALB/c mice.

Biochem/physiol Actions

Staphylococcal enterotoxin B (SEB) is a superantigen that binds to class II MHC molecules on antigen presenting cells. It stimulates large populations of T cells resulting in acute toxic shock syndrome. It is resistant to denaturation and causes outbreaks of food poisoning that includes emetic responses, diarrhea and GI inflammation.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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