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S9059 Sigma

Streptavidin−Gold from Streptomyces avidinii

~10 nm nominal (colloidal gold), buffered aqueous glycerol solution

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Properties

Related Categories Antibodies, Biotin/Avidin, Streptavidin Reagents, Supplementary Products
form   buffered aqueous glycerol solution
concentration   ~2.5 A520 units/mL
nominal   ~10 nm (colloidal gold)
mean particle size   8-12 nm (monodisperse)
conjugate   gold conjugate
storage temp.   2-8°C

Description

Application

For detection of biotinylated compounds.

Strepavidin-collodial gold conjugate is a component of streptavidin/colloidal gold-biotin detection systems use in immunoblotting, light microscopy, and immunoelectron microscopy applications. It is commonly used in postembedding immunocytochemistry.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.2, containing 1% BSA, 20% glycerol, and 15 mM sodium azide

General description

Strepavidin-collodial gold conjugate binds to molecules, such as primary antibodies, that have been previously conjugated with biotin.

Streptavidin is a protein produced by the bacterium Streptomyces avidinii. It has four binding sites for biotin as does avidin. Both have been used extensively as probes in immunochemical systems, conjugated to antibodies, enzymes, fluorochromes and gold. Avidin is highly basic (pI ~ 10.5), compared to streptavidin (pI ~ 5-6). Avidin has a slightly higher binding capacity for biotin than streptavidin does, but it has glycosylated side chains which give higher backgrounds in blotting techniques than does streptavidin, which is not a glycoprotein. Streptavidin is more resistant than avidin to dissociation into subunits by guanidinium chloride. Streptavidin may be conjugated to colloidal gold.

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Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

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