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S9415 Sigma

Streptavidin−Maleimide from Streptomyces avidinii

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Properties

Related Categories Antibodies, Biotin/Avidin, Streptavidin Reagents, Supplementary Products
extent of labeling   4-10 mol maleimide per mol Streptavidin
suitability   suitable for direct conjugation to compounds containing free sulfhydryl groups
storage temp.   −20°C

Description

Preparation Note

Streptavidin, from Streptomyces avidinii, activated with maleimidocaproic acid N-hydroxysuccinimide ester.

Physical form

Lyophilized powder containing sodium citrate.

Application

Maleimide Activated Streptavidin is used to prepare streptavidin-protein conjugates with proteins, peptides and other molecules that contain a free sulfhydryl (-SH) group. Activated streptavidin presents an available maleimide group that reacts with sulfhydryl-containing molecules.

General description

Streptavidin conjugated compounds may be used in avidin/strepavidin:biotin-based detection systems.

Streptavidin is a protein produced by the bacterium Streptomyces avidinii. It has four binding sites for biotin, as does avidin. Both have been used extensively as probes in immunochemical systems, conjugated to antibodies, enzymes or fluorochromes. However, avidin is highly basic (pI ~ 10.5), compared to streptavidin (pI ~ 5-6). Avidin has a slightly higher binding capacity for biotin than streptavidin does, but it has glycosylated side chains which give higher backgrounds in blotting techniques than does streptavidin, which is not a glycoprotein. Streptavidin is more resistant than avidin to dissociation into subunits by guanidinium chloride.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

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Peer-Reviewed Papers
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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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