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S9555 Sigma

Monoclonal Anti-E-Selectin (CD62E) antibody produced in mouse

~2 mg/mL, clone 1.2B6, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-E-Selectin, Anti-ELAM-1, Monoclonal Anti-CD62E

Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
species reactivity   pig, human
application(s)   capture ELISA: suitable
  flow cytometry: suitable
  immunohistochemistry (frozen sections): suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  indirect immunofluorescence: 1-2 μg/mL using using 293-T cells transfected with E-selectin fusion protein
  western blot: suitable
clone   1.2B6, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 100-115 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... SELE(6401)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

TNF-α activated human endothelial cells.

Antigen Background

CD62E (E-Selectin) surface antigen is a glycoprotein and member of the selectin family of adhesion receptors. It is expressed by activated human endothelial cells and is detectable in the plasma.

Physical form

Solution, 0.2 μm filtered, in 0.01 M phosphate buffered saline, pH 7.4.

Specificity

Reacts specifically with human CD62E (E-Selectin, ELAM-1) expressed on the surface of activated endothelial cells. The antibody may weakly cross-react with recombinant hman P-Selectin and human platelet P-Selectin. Cross-reactivity has been observed with porcine E-Selectin. Monoclonal Anti-human E-Selctin inhibits adhesion of neutrophils, eosinophils, and a skin-homing T-cell subpopulation to activated endothelial cells and CD62E-transfected cells. 5th Workshop: code no. E 040.

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