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S9751 Sigma

Sulfatase from Helix pomatia

Type H-2, aqueous solution, ≥2,000 units/mL

Synonym: Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate and PTM Analysis,
type   Type H-2
form   aqueous solution
secondary activity   ≥100,000 units/mL β-glucuronidase (at pH 5.0)
concentration   ≥2,000 units/mL
shipped in   wet ice
storage temp.   2-8°C

Description

General description

Sequential action of sulfatase from Helix pomatia and β-O-glucosidase from Caldicellulosiruptor saccharolyticus on glucosinolates allows synthesis of thiohydroximates. Sulfatase from Helix pomatia hydrolyzes β-naphthyl sulfate much faster than α-naphthyl sulfate.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).

Application

Sulfatase from Helix pomatia is often used to remove sulfate groups from carbohydrates.

Price and Availability


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Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Protocols

Enzymatic Assay of Sulfatase

This procedure applies to all products that have a specification for Sulfatase activity, except Sulfatase from Aerobacter aerogenes, Sigma-Aldrich Product Number S1629.
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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