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S9751 Sigma

Sulfatase from Helix pomatia

Type H-2, aqueous solution, ≥2,000 units/mL

Synonym: Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate and PTM Analysis,
type   Type H-2
form   aqueous solution
secondary activity   ≥100,000 units/mL β-glucuronidase (at pH 5.0)
concentration   ≥2,000 units/mL
shipped in   wet ice
storage temp.   2-8°C

Description

General description

Sequential action of sulfatase from Helix pomatia and β-O-glucosidase from Caldicellulosiruptor saccharolyticus on glucosinolates allows synthesis of thiohydroximates. Sulfatase from Helix pomatia hydrolyzes β-naphthyl sulfate much faster than α-naphthyl sulfate.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).

Application

Sulfatase from Helix pomatia is often used to remove sulfate groups from carbohydrates.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Protocols

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

This procedure may be used for determination of Sulfatase activity in products, except for Sulfatase products from Aerobacter aerogenes (Product Number S1629).
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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