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SAB3500101 Sigma

Anti-SKA1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Spindle and kinetochore associated complex subunit 1

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies with Corresponding Blocking Peptides, Primary Antibodies, R-S,
species reactivity   mouse, rat, human
application(s)   indirect ELISA: suitable
  western blot: suitable
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... SKA1(220134)
biological source   rabbit
conjugate   unconjugated
NCBI accession no.   Q96BD8
UniProt accession no.   Q96BD8

Description

Immunogen

SKA1 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of human SKA1.

Antigen Background

Upon entry into mitosis, the cell’s microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing.

Physical form

Supplied at approx. 1 mg/mL in phosphate buffered saline containing 0.02% sodium azide.

Linkage

The action of this antibody can be blocked using blocking peptide SBP3500101.

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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SBP3500101 SKA1 Blocking Peptide for SAB3500101

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