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SAB3500103 Sigma

Anti-SKA3 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-RAMA1, Anti-Spindle and kinetochore associated complex subunit 3

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies with Corresponding Blocking Peptides, Primary Antibodies, R-S,
species reactivity   human, mouse, rat
application(s)   indirect ELISA: suitable
  western blot: suitable
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... SKA3(221150)
biological source   rabbit
conjugate   unconjugated
NCBI accession no.   NP_659498
UniProt accession no.   Q8IX90

Description

Immunogen

SKA3 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of human SKA3.

Antigen Background

Upon entry into mitosis, the cell’s microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.

Physical form

Supplied at approx. 1 mg/mL in phosphate buffered saline containing 0.02% sodium azide.

Linkage

The action of this antibody can be blocked using blocking peptide SBP3500103.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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SBP3500103 SKA3 Blocking Peptide for SAB3500103

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