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SAB3500120 Sigma

Monoclonal Anti-ORAI3 antibody produced in mouse

clone 2H2G9, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-Calcium release-activated calcium channel protein 3, Anti-TMEM142C, Anti-Transmembrane protein 142C

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Properties

Related Categories Alphabetical Index, Antibodies, O, Primary Antibodies
biological source   mouse
antibody form   purified immunoglobulin
clone   2H2G9, monoclonal
form   buffered aqueous solution
mol wt   predicted mol wt 32 kDa
species reactivity   rat, human
application(s)   immunofluorescence: suitable
  immunohistochemistry: suitable
  indirect ELISA: suitable
  western blot: suitable
conjugate   unconjugated
UniProt accession no.   Q9BRQ5
shipped in   dry ice
storage temp.   −20°C

Description

Antigen Background

Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2 (5). Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE; these may represent post-translationally modified ORAI 3.

Immunogen

raised against a 19 amino acid peptide from near the carboxy terminus of human ORAI3.

Physical form

Supplied at approx. 1 mg/mL in phosphate buffered saline containing 0.02% sodium azide.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

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