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SAB4200253 Sigma

Monoclonal Anti-triMethyl-Histone H4 (triMe-Lys20) antibody produced in mouse

~1.0 mg/mL, clone 6F8-D9, purified immunoglobulin

Synonym: Anti-H4K20me3, Anti-HIST4H4

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Histones, Epigenetics, Primary Antibodies,
species reactivity   human, mouse, rat
application(s)   indirect immunofluorescence: 5-10 μg/mL using cultured human HeLa cells
  western blot: 1-2 μg/mL using histones isolated from human HeLa cells
clone   6F8-D9, monoclonal
concentration   ~1.0 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   mol wt ~14 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... HIST4H4(121504)
mouse ... Hist1h4(319156)
rat ... Hist2h4(295277)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

hybridoma 6F8-D9 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide containing trimethylated Lys20 of human Histone H4

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Price and Availability

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