• USA Home
  • SAB4504169 - Anti-phospho-DNA-PK (pSer2056) antibody produced in rabbit

EMAIL THIS PAGE TO A FRIEND
SAB4504169 Sigma

Anti-phospho-DNA-PK (pSer2056) antibody produced in rabbit

affinity isolated antibody

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins at Phosphoserine (pSer),
species reactivity   human, mouse, rat
application(s)   immunohistochemistry: suitable
  indirect ELISA: suitable
clone   polyclonal
concentration   ~1 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 469 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... PRKDC(5591)
biological source   rabbit
conjugate   unconjugated
NCBI accession no.   P78527

Description

Immunogen

The antiserum was produced against synthesized peptide derived from human DNA-PK around the phosphorylation site of Ser2056.

General description

Protein kinase, DNA-activated, catalytic polypeptide (PRKDC), also known as DNAPK, is encoded by the gene mapped to human chromosome 8q11. PRKDC is characterized by a large catalytic polypeptide and DNA-binding component comprised of the Ku70 and Ku80 heterodimer, that targets DNA-PKc, to DNA.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application

Anti-phospho-DNA-PK (pSer2056) antibody produced in rabbit has been used in immunofluorescence analysis.

Biochem/physiol Actions

Protein kinase, DNA-activated, catalytic polypeptide (PRKDC), also known as DNAPK, plays an essential role in the colorectal cancer (CRC) cell proliferation/survival. Therefore, it can be used as a potential prognostic biomarker and a therapeutic target for CRC. Decreased expression of PRKDC influences apoptosis partially by inhibiting AKT activation. Overexpression of activated DNAPK elevates the expression of rearranged during transfection (RET), which is a proto-oncogene involved in the development of medullary thyroid cancer (MTC). DNAPK plays a crucial role in non-homology end joining (NHEJ) pathway. Downregulation of DNAPK enhances anticancer drug induced DNA damage repair in osteosarcoma cell line MG63.

Price and Availability


Antibody Explorer

Explore with Confidence
Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  1
Protocols & Articles

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?