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SAB5300456 Sigma

Monoclonal Anti-GCK antibody produced in mouse

clone 4G6, ascites fluid

Synonym: GK, GLK, HHF3, HK4, HKIV

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Properties

Related Categories Alphabetical Index, Antibodies, G-GC, Primary Antibodies
species reactivity   human
application(s)   direct ELISA: 1:10,000
  western blot: 1:500-1:2,000
clone   4G6, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   mol wt 52 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... GCK(2645)
biological source   mouse
conjugate   unconjugated
NCBI accession no.   NM_000162.3

Description

Immunogen

Purified recombinant fragment of human GCK expressed in E.coli.

General description

The gene encoding glucokinase (GCK) is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The enzyme is expressed as a monomer and possesses one glucose-binding site. The gene encoding GCK is localized on human chromosome 7.

Antigen Background

Mouse monoclonal antibody raised against GCK

Physical form

Ascitic fluid containing 0.03% sodium azide.

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Biochem/physiol Actions

Glucose is phosphorylated to glucose-6-phosphate by glucokinases. The main function of glucokinase (GCK) is to regulate carbohydrate metabolism. The enzyme triggers the release of insulin in β-cells of pancreas. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity. Mutation in the gene encoding this protein is associated with a rare form of diabetes and the protein may also have a role in type 2 diabetes.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Peer-Reviewed Papers
15

References

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