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SCA20 Sigma

Sucrose Assay Kit

sufficient for 20 assays

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Description

Application

This kit is for the quantitative, enzymatic determination of sucrose in food and other materials. Sucrose is hydrolyzed to glucose and fructose by invertase. Glucose and fructose are phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to sucrose concentration.

General description

Sucrose is a disaccharide, naturally occurring carbohydrate found in plants and a major product during photosynthesis.

Price and Availability


All labs need water

Standard component

Description

Product #

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glucose assay reagent 50 mL SDS    
sucrose assay reagent 1 mL/vial SDS    
sucrose standard 500 mg SDS    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Enzymatic Food Analysis

Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. Sigma-Aldrich offers a wide...
Jvo Siegrist
Product Manager Microbiology ivo.siegrist@sial.com, AnalytiX Volume 9 Article 2
Keywords: Absorption, Clinical, Degradations, Diabetes, Enzyme activity, Food Analysis, Nutrition, Obesity, Sample preparations

Peer-Reviewed Papers
15

References

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