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SHC001V Sigma

MISSION® pLKO.1-puro Empty Vector Control Transduction Particles

Contains no shRNA insert

Synonym: MISSION® TurboGFP® Control Transduction Particles

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Description

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

General description

When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


All labs need water
Safety & Documentation
Protocols & Articles

Articles

Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library

Betsy Boedeker,* Gregory A. Wemhoff, Andrea Spencer, Roger Chiu, Suzanne Miller, Henry George, and Edward J. Weinstein
Betsy Boedeker, Gregory A. Wemhoff, Andrea Spencer,Roger Chiu, Suzanne Miller, Henry George and Edward J. Weinstein
LSI Volume 7 Article 1
Keywords: Amplification, Antibiotics, Cellular processes, Drug discovery, Functional genomics, Gene expression, Genomics, Molecular biology, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Reductions, Sequences, Transcription, Transduction, Vectors

Protocols

Lentiviral Transduction Protocol

Prepare mammalian cells so that they are growing exponentially and are no more than 70-80% confluent before transduction.
Keywords: Cell culture, Gene expression, Molecular biology, Transduction

Peer-Reviewed Papers
15

References

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SHC002 MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA, Targets no known mammalian genes
SHC003 MISSION® pLKO.1-puro-CMV-TurboGFP Positive Control Plasmid DNA, Green fluorescent protein marker to monitor transduction efficiency
SHC002V MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, Targets no known mammalian genes
SHC003V MISSION® pLKO.1-puro-CMV-TurboGFP Positive Control Transduction Particles, Green fluorescent protein marker to monitor transduction efficiency
SHC004 MISSION® pLKO.1-puro TurboGFP shRNA Control Plasmid DNA, shRNA sequence targeting tGFP
SHC004V MISSION® pLKO.1-puro TurboGFP shRNA Control Transduction Particles, shRNA sequence targeting tGFP
SHM01 MISSION® ExpressMag® Super Magnetic Kit, Increases transduction efficiency Enhance your lentiviral transduction!
SHM02 MISSION® ExpressMag® 96-Well Magnetic Kit, Increases transduction efficiency Enhance your lentiviral transduction in 96-well plate format!
SHC016V MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles, Targets no known genes from any species

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