MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Plasmid DNA
Green fluorescent protein marker to monitor transduction efficiency
DOWNLOAD MSDS (PDF)Synonym: MISSION® Control Vectors
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MDL number MFCD07785395
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Popular Documents: Bulletin (PDF)
Properties
| Related Categories | Cell Biology, Functional Genomics and RNAi, General Stem Cell Biology, MISSION Controls in DNA Format, MISSION shRNA, |
| shipped in | dry ice |
| storage temp. | −20°C |
Description
Frequently Asked Questions
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Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
General description
The MISSION TurboGFP Control Vector is an 8347 base pair lentivirus plasmid vector that contains a gene encoding TurboGFP, under the control of the CMV promoter. The TurboGFP Control Vector is useful as a positive transfection/transduction control in experiments using the MISSION shRNA library clones. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/m
MISSION is a registered trademark of Sigma-Aldrich Co. LLC
TurboGFP is a trademark of Evrogen Co.
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