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SHC003V Sigma

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles

Green fluorescent protein marker to monitor transduction efficiency

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Synonym: MISSION® TurboGFP® Control Transduction Particles

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Properties

Related Categories Cell Biology, Functional Genomics and RNAi, General Stem Cell Biology, MISSION Controls in Lentiviral Format, MISSION shRNA Controls,
application(s)   capture ELISA: 106 TU/mL using p24
shipped in   dry ice
storage temp.   −70°C

Description

General description

When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION TurboGFP™ Control Transduction Particles are a critical positive control to monitor transduction efficiency.

This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest.

TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from the copepoda Pontellina plumata. The TurboGFP transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-CMV-TurboGFP (SHC003).

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

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