MISSION® pLKO.1-puro TurboGFP™ shRNA Control Plasmid DNA
shRNA sequence targeting tGFP
DOWNLOAD MSDS (PDF)Synonym: MISSION® Control Vectors
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MDL number MFCD07785395
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Popular Documents: Bulletin (PDF)
Properties
| Related Categories | Functional Genomics and RNAi, MISSION Controls in DNA Format, MISSION shRNA, MISSION shRNA Controls |
| shipped in | dry ice |
| storage temp. | −20°C |
Description
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
General description
The MISSION TurboGFP shRNA Control Vector is a 7,087 base pair lentivirus plasmid vector that contains an shRNA sequence targeting TurboGFP.1 The TurboGFP shRNA Control Vector is useful as a positive knockdown control in experiments using the MISSION TurboGFP positive control vector or in cell lines expressing TurboGFP. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.2,3 The TurboGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/m
MISSION is a registered trademark of Sigma-Aldrich Co. LLC
TurboGFP is a trademark of Evrogen Co.
Price and Availability
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