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SHC005 Sigma

MISSION® pLKO.1-puro eGFP shRNA Control Plasmid DNA

shRNA sequence targeting eGFP

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Synonym: MISSION® Control Vectors

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Properties

Related Categories Functional Genomics and RNAi, MISSION Controls in DNA Format, MISSION shRNA Controls, shRNA
shipped in   dry ice
storage temp.   −20°C

Description

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

General description

The MISSION eGFP shRNA Control Vector is a 7,091 base pair lentivirus plasmid vector that contains an shRNA sequence targeting eGFP (GenBank Accession No. pEGFP U55761).1 The eGFP shRNA Control Vector is useful as a positive knockdown control in experiments using cell lines expressing eGFP.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids2, MISSION Lentiviral Packaging Mix (Prod. No. SHP001). The eGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

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