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SHC010 Sigma

MISSION® pLKO.1-puro-CMV-TagCFP™ Positive Control Plasmid DNA

Contains a gene encoding TagCFP

DOWNLOAD MSDS (PDF)

Synonym: MISSION® Control Vectors

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Properties

Related Categories Functional Genomics and RNAi, MISSION Controls in DNA Format, MISSION shRNA Controls, shRNA
shipped in   dry ice
storage temp.   −20°C

Description

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.
The MISSION TagCFP Control Vector is an 8597 base pair lentivirus plasmid vector that contains a gene encoding TagCFP, under the control of the CMV promoter. The CMV-TagCFP Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones.

General description

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, we recommend producing self-inactivating replication incompetent viral particles in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The CMV-TagCFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

TagCFP is a trademark of Evrogen Co.

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