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SHC011
Sigma
MISSION® pLKO.1-
MISSION® pLKO.1-puro-CMV-TagYFP™ Positive Control Plasmid DNA
Contains a gene encoding TagYFP
DOWNLOAD MSDS (PDF)Synonym: MISSION® Control Vectors
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MDL number MFCD07785395
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Popular Documents: Bulletin (PDF)
Properties
| Related Categories | Functional Genomics and RNAi, MISSION Controls in DNA Format, MISSION shRNA Controls, shRNA |
| shipped in | dry ice |
| storage temp. | −20°C |
Description
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
The MISSION TagYFP Control Vector is an 8599 base pair lentivirus plasmid vector that contains a gene encoding TagYFP, under the control of the CMV promoter. The CMV-TagYFP Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones.
General description
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, we recommend producing self-inactivating replication incompetent viral particles in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The CMV-TagYFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/m
MISSION is a registered trademark of Sigma-Aldrich Co. LLC
TagYFP is a trademark of Evrogen Co.
Price and Availability
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