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SHC011V Sigma

MISSION® pLKO.1-puro-CMV-TagYFP™ Positive Control Particles

Contains a gene encoding TagYFP

DOWNLOAD MSDS (PDF)

Synonym: MISSION® TurboGFP® Control Transduction Particles

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Properties

Related Categories Functional Genomics and RNAi, MISSION Controls in Lentiviral Format, MISSION shRNA, MISSION shRNA Controls
application(s)   capture ELISA: 106 TU/mL using p24
shipped in   dry ice
storage temp.   −70°C

Description

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION TagYFP Control Transduction Particles are a positive control to monitor transduction efficiency. The MISSION TagYFP Control Transduction Particles are produced from the sequence-verified 8599 base pair lentivirus plasmid, pLKO.1-puro-CMV-TagYFP (SHC011).

This construct aids in interpretation of experimental design and results by providing a yellow fluorescent protein marker for monitoring success in transduction of your cells of interest.

General description

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

TagYFP is a trademark of Evrogen Co.

Price and Availability

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