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SHPH01 - MISSION^® LentiPlex^® Human Pooled shRNA Library

SHPH01 Sigma

MISSION^® LentiPlex^® Human Pooled shRNA Library

For Rapid, Convenient Genome-wide shRNA Screens

Popular Documents: Bulletin (PDF)

Properties

Related Categories	Functional Genomics and RNAi, MISSION shRNA, Pooled shRNA Screening More...
application(s)	capture ELISA: 5 × 10⁸ TU/mL using p24
shipped in	dry ice
storage temp.	−70°C

Description

Other Notes

Deconvolution of shRNA Pools
Sigma′s deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.

• Next-generation sequencing of clonesgives a precise number of individual clone occurrence within a pooled shRNA sample
• Comprehensive, reproducible results from pooled shRNA screens
• Statistically robust and information-rich data

Contact your local Sigma sales representative for more information or submit an inquiry to MISSION RNAi

General description

MISSION LentiPlex Pooled Libraries bring the power of the MISSION TRC shRNA collection together with Sigma¢s lentiviral manufacturing expertise to enable genome-wide RNAi screens at your bench-top. The LentiPlex pooled system provides enhanced delivery and long-term gene silencing in non-dividing and primary cell lines allowing unlimited discovery potential. The convenient format now makes rapid whole-genome RNAi screens accessible to any researcher with minimal reagent, time or capital equipment investment.

The MISSION LentiPlex Human shRNA Pooled Library is a genome-wide lentiviral pool produced using a proprietary process. The library consists of over 75,000 shRNA constructs from the TRC collection targeting 15,000+ human genes. Each library is tested for shRNA representation before product release to ensure robust library coverage. The library is provided in ready-to-use lentiviral format at titers of at least 5 x 108 TU/ml via p24 assay and is pre-divided into ten subpools of approximately 8,000 shRNA constructs each. Amplification and sequencing primers are also provided for downstream target identification.

The library is pre-divided into ten subpools of approximately 8,000 shRNA constructs each. Each library is enough material for six screens at 100X representation.

Please visit sigma.com/lentiplex to learn more and see example data and uses.

Legal Information

LentiPlex is a registered trademark of Sigma-Aldrich Co. LLC

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Related Papers

Forward RNAi screens in human stem cells. Karlsson, C., et al. Methods Mol. Biol. 650, 29-43, (2010)

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MISSION^® LentiPlex^® Human Pooled shRNA Library

For Rapid, Convenient Genome-wide shRNA Screens

Properties

Description

Other Notes

General description

Legal Information

Price and Availability

Related Papers

Documents

Safety Information

Articles

Related Content

Papers

related product

required but not provided

Technical Service:

Bulk Ordering & Pricing:

EMAIL THIS PAGE TO A FRIEND

MISSION® LentiPlex® Human Pooled shRNA Library

For Rapid, Convenient Genome-wide shRNA Screens

Properties

Description

Other Notes

General description

Legal Information

Price and Availability

Related Papers

Documents

Safety Information

Articles

Related Content

Papers

related product

required but not provided

Technical Service:

Bulk Ordering & Pricing:

MISSION^® LentiPlex^® Human Pooled shRNA Library