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SIALICQ Sigma

Sialic Acid Quantitation Kit

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Description

Application

Contains all reagents to quantitate the amount of N-acetylneuraminic acid, either free, or in glycoproteins, cell surface glycoproteins, polysialic acids and capsular polysaccharides (consisting only of polysialic acid).

General description

The Sialic Acid Quantitation Kit provides a rapid and accurate determination of total N-acetylneuraminic acid (NANA; sialic acid). NANA is released from glycoconjugates using neuraminidase. The kit uses α(2→3,6,8,9) neuraminidase to cleave all NANA linkages, including α(2→8) and α(2→9) linkages, as well as branched NANA, for the most accurate determination of total sialic acid content.

Quantity

Sufficient reagents are provided to perform 25 reactions, including control samples. Each reaction will measure 1-200 nmoles of NANA.

Price and Availability


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Description

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N-Acetylneuraminic acid aldolase 25 μL SDS    
N-Acetylneuraminic acid 200 μL SDS    
α(2→3,6,8,9)-Neuraminidase 25 μL SDS    
Fetuin, bovine .5 mg SDS    
Lactic dehydrogenase 25 μL SDS    
β-NADH disodium salt 3 vials SDS    
Tris-HCl, pH 7.5 1 mL SDS    
Sialidase buffer 500 μL SDS    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

NMR Analysis of Glycans

Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field. This technique is non-destructive and can provide full structural info...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Cancer, Digestions, Electrophoresis, Nuclear magnetic resonance spectroscopy, Separation, Spectroscopy

Peer-Reviewed Papers
15

References

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