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SRP0115 Sigma

Sirtuin 1 human

recombinant, expressed in E. coli, ≥60% (SDS-PAGE)

Synonym: SIR2, SIRT1, Sir2A, sir2-like 1

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Properties

Related Categories Deacetylases, Epigenetics, Histone Modifying Enzymes, Molecular Biology, Proteins and Peptides for Epigenetic Research More...
recombinant   expressed in E. coli
assay   ≥60% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 87.2 kDa
NCBI accession no.   NM_012238
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... SIRT1(23411)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Human Sirtuin 1, GenBank Accession No. NM_012238), amino acids 193-741 with N-terminal ST tag, MW = 87.2 kDa, expressed in E. coli expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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