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SRP0116 Sigma

Sirtuin 2 human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym: SIR2L2, SIRT2, sir2-like 2

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Properties

Related Categories Deacetylases, Epigenetics, Histone Modifying Enzymes, Molecular Biology, Proteins and Peptides for Epigenetic Research More...
recombinant   expressed in E. coli
assay   ≥80% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 35.5 kDa
NCBI accession no.   NM_030593
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... SIRT2(22933)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Biochem/physiol Actions

Silent information regulator 2 (Sir2) acts as a nicotine adenine dinucleotide (NAD) dependent deacetylase and is implicated in various biological processes such as regulation of transcriptional repression, recombination, the cell-division cycle, microtubule organization, and cellular responses to DNA-damaging agents. In addition, sirtuins also regulate the process involved in aging. Sir2 plays a vital role in mammalian metabolic homeostasis and is considered to be a potent therapeutic target for the treatment of type 2 diabetes. Sir2 has a crucial role in reduction of microglial activation and brain inflammation. Therefore, loss of Sir2 activity increases the risk of susceptibility to neurodegenerative diseases.

General description

Silent information regulator 2 (Sir2) or sirtuin 2 is encoded by the gene mapped to human chromosome 19q13.2. It belongs to class Ib of sirtuin protein family. Sir2 is characterized with a catalytic domain containing two different domains that bind NAD and the acetyl-lysine substrate, respectively. Apart from this, it also constitutes variable NH2 and COOH-terminal domains involved in the regulation of subcellular localizations and catalytic activity. sirtuin 2 is mainly expressed in cytosol but its migration between cytosol and nucleus facilitates the deacetylation of both α-tubulin and histones.
Human Sirtuin 2, GenBank Accession No. NM_030593, amino acids 13-319 with C-terminal His tag, MW = 35.5kDa, expressed in Escherichia coli expression system.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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