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SRP0122 Sigma

LSD1 Active human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonym: Amine oxidase (flavin containing) domain 2 (AOF2), BRAF35-HDAC complex protein BHC110, KIAA0601, Lysine (K)-specific demethylase 1 (LSD1), Lysine-specific histone demethylase 1A (KDM1A)

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Properties

Related Categories Demethylases, Epigenetics, Histone Modifying Enzymes, Molecular Biology, Proteins and Peptides for Epigenetic Research More...
recombinant   expressed in E. coli
assay   ≥70% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 103 kDa
NCBI accession no.   NM_015013
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... KDM1A(23028)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Human LSD1, AOF2, amine oxidase, flavin containing, domain 2, GenBank Accession No. NM_015013, amino acids 158-end with N-terminal ST tag, MW = 103 kDa, expressed in E. coli expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Price and Availability

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Modulators and Proteins for Methylation

DNA methyltransferases are a family of enzymes that catalyze the transfer of a methyl group to DNA. There are five related DNA cytosine-5-methyltransferases (DNMTs) that transfer a methyl group from ...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 7–9
Keywords: Gene expression, Methylations, PAGE

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