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SRP0123 Sigma

JMJD2C Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym: GASC1, Gene amplified in squamous cell carcinoma 1 protein, JHDM3C, KIAA0780, Lysine-specific demethylase 4C (KDM4C)

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Properties

Related Categories Demethylases, Epigenetics, Histone Modifying Enzymes, Molecular Biology, Proteins and Peptides for Epigenetic Research More...
recombinant   expressed in baculovirus infected insect cells
assay   ≥80% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 69 kDa
NCBI accession no.   BC143571
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... KDM4C(23081)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Biochem/physiol Actions

JMJD2C (Jumonji domain containing), also known as KDM4C (lysine demethylase 4C), is a type of KDM4 demethylases that are responsible for the demethylation of H3K9me2/3, H3K36me3/2, and H1.4K26me3/2. This is accomplished through a hydroxylation reaction requiring the cofactors Fe(II) and 2-oxoglutarate (2-OG). These proteins show heightened expression and activity in multiple types of cancers, and hence, are also classified as oncogenes. KDMA4 family members are capable of coregulating hormone receptors, and hence, are implicated in the progression of hormone dependent cancers, such as breast and prostate cancer. JMJD2C is essential for regulating the expression of growth promoting proteins and the survival factor FRA1 (Fos-related antigen 1) and cyclin D1, and BCL2 (B-cell lymphoma) respectively. The expression of JMJD2C is increased in colon cancer cell, where it interacts with the oncogenic protein β-catenin, the up-regulation of which is essential for the pathogenesis of colon cancer.

General description

JMJD2C (Jumonji domain containing), also known as KDM4C (lysine demethylase 4C), is an H3K9me3 demethylase belonging to the conserved family of H3K9me3 erasers, KDM4 demethylases. KDM4 family was first identified in 2006, and is composed of six members namely KDM4A, -B, -C, -D, -E, and -F. This protein predominantly resides at H3K4me3-containing promoter region. JMJD2C protein has multiple reader domains, including a hybrid tandem tudor domain (TTD) and two PHDs (plant homeodomain) of unknown function.
Human JMJD2C, GenBank Accession No. BC143571) (amino-acids 2-372) with N-terminal ST tag, MW = 69kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Price and Availability

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles

Articles

Modulators and Proteins for Methylation

DNA methyltransferases are a family of enzymes that catalyze the transfer of a methyl group to DNA. There are five related DNA cytosine-5-methyltransferases (DNMTs) that transfer a methyl group from ...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 7–9
Keywords: Gene expression, Methylations, PAGE

Peer-Reviewed Papers
15

References

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