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SRP0124 Sigma

CoREST human

recombinant, expressed in E. coli, ≥60% (SDS-PAGE)

Synonym: RCOR1, REST corepressor 1

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Demethylases, Epigenetics, Histone Modifying Enzymes,
recombinant   expressed in E. coli
assay   ≥60% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 20 kDa
NCBI accession no.   NM_015156
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... RCOR1(23186)

Description

Application

Useful for the stimulation of LSD1 enzyme activity

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Biochem/physiol Actions

CoREST associates with REST (RE1-silencing transcription factor), the histone deacetylases 1 and 2 (HDAC1/2), and LSD1 (lysine-specific histone demethylase 1A). This complex is involved in chromatin remodeling for changing gene expression. The LSD1/CoREST/REST compressor complex is also required for the expression of Herpes Simplex virus genes. LSD1 along with CoREST is responsible for the oxidative demethylation of the H3 histone (mono- and di-methylated Lysine4 residue). Deletions in the CoREST gene results in unfavorable clinical outcomes in DLBCL (diffuse large B-cell lymphoma) patients. It might also participate in the epigenetic control of blood cell development.

General description

CoREST (also referred to as RCOR1 (REST corepressor 1)) mRNA is ubiquitously expressed in all tissues. It is a corepressor of the RE1-silencing transcription factor REST. In nonneuronal cells, the transcription factor binds to RE1 neuron-restrictive silencer elements and suppresses gene expression. CoREST is a component of the BRAF35 (BRCA2-associated factor 35)–histone deacetylase complex. The RCOR1 gene is mapped to human chromosome 14q32.
Human recombinant CoREST, GenBank Accession No. NM_015156), amino acids 305-end, with N-terminal His tag, MW = 20kDa, expressed in Escherichia coli expression system.

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All labs need water
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles

Articles

Huntington's Disease

Huntington's disease (HD) is an autosomal dominant, late-onset neurodegenerative disorder characterized by a selective neuronal cell death in the cortex and striatum leading to cognitive dysfunction,...
Carolyn L. Crankshaw
BioFiles v7 n2, 2011, 9–14
Keywords: Apoptosis, Huntington Disease, Transcription

Modulators and Proteins for Methylation

DNA methyltransferases are a family of enzymes that catalyze the transfer of a methyl group to DNA. There are five related DNA cytosine-5-methyltransferases (DNMTs) that transfer a methyl group from ...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 7–9
Keywords: Gene expression, Methylations, PAGE

Peer-Reviewed Papers
15

References

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