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SRP0126 Sigma

DNMT1 Active human

recombinant, expressed in baculovirus infected insect cells, ≥50% (SDS-PAGE)

Synonym: CXXC-type zinc finger protein 9, DNA (cytosine-5-)-methyltransferase 1, DNA MTase HsaI, MCMT

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Properties

Related Categories Cell Culture, DNMTs, Epigenetics, Growth Factors and Cytokines, Histone Modifying Enzymes,
recombinant   expressed in baculovirus infected insect cells
assay   ≥50% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 211 kDa
NCBI accession no.   NM_001130823
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... DNMT1(1786)

Description

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme required to methylate 1 pmol of DNA substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Application

DNMT1 (DNA methyltransferase 1) has been used for RNA electrophoretic mobility shift assay (REMSA) to determine the role of extra-coding RNAs (ecRNAs) in controlling neuronal DNA methylation, through interaction with DNA methyltransferases.

Biochem/physiol Actions

DNMT1 (DNA methyltransferase 1) is the predominant enzyme that is responsible for maintaining the level of DNA methylation. It is responsible for replicating CpG methylation patterns from parent to daughter DNA strands, thus, creating heritable methylation signatures through cell division. It, therefore, maintains epigenetic state of DNA. Abnormalities in the expression of this protein are linked with the progression and prognosis of multiple cancers, such as hepatocellular carcinoma, pancreatic, lung and bladder cancers. Up-regulation of this protein in GC (gastric cancer) with less differentiation, advanced stage and increased rate of mortality. Over-expression of this protein is linked with poor prognosis in malignant lymphoma, renal cell carcinoma, pancreatic cancer, and bladder cancer.

General description

DNMT1 (DNA methyltransferase 1) is a member of the DNMT enzyme family which also includes DNMT2, DNMT3A, and DNMT3B. It is a multidomain protein composed of 1616 amino acids. It has a methyltransferase domain in its C-terminal, which shows sequence homology to bacterial methyltransferases.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Modulators and Proteins for Methylation

DNA methyltransferases are a family of enzymes that catalyze the transfer of a methyl group to DNA. There are five related DNA cytosine-5-methyltransferases (DNMTs) that transfer a methyl group from ...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 7–9
Keywords: Gene expression, Methylations, PAGE

Peer-Reviewed Papers
15

References

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