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SRP0127 Sigma

DNMT2 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym: DNA (cytosine-5-)-methyltransferase 2, PUMET, RNMT1, tRNA (cytosine-5-)-methyltransferas, tRNA aspartic acid methyltransferase 1

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Properties

Related Categories Cell Culture, DNMTs, Epigenetics, Growth Factors and Cytokines, Histone Modifying Enzymes,
recombinant   expressed in baculovirus infected insect cells
assay   ≥80% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 71 kDa
NCBI accession no.   NM_004412
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... TRDMT1(1787)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Biochem/physiol Actions

DNA (cytosine-5-)-methyltransferase 2 (DNMT2) methylates the cytosine-38 residue of the aspartic acid transfer RNA (tRNA-Asp). It has been shown to interact with the anticodon stem and loop. The protein also functions in cellular physiology and stress response. It is significantly expressed in cancers.

General description

DNA (cytosine-5-)-methyltransferase 2 (DNMT2) is a highly conserved protein which is part of the DNA methyltransferase family. It possesses a conserved cysteine residue in its catalytic pocket. The gene encoding it is localized on human chromosome 10p14->p12.

Price and Availability

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles

Articles

Modulators and Proteins for Methylation

DNA methyltransferases are a family of enzymes that catalyze the transfer of a methyl group to DNA. There are five related DNA cytosine-5-methyltransferases (DNMTs) that transfer a methyl group from ...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 7–9
Keywords: Gene expression, Methylations, PAGE

Peer-Reviewed Papers
15

References

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