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SRP0132 Sigma

EZH2 / EED / SUZ12 human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

Synonym: ENX-1, EZH2, Enhancer of zeste homolog 2, KMT6A, Lysine N-methyltransferase 6A



Related Categories Epigenetics, Histone Modifying Enzymes, Methyltransferases, Molecular Biology, Proteins and Peptides for Epigenetic Research More...
recombinant   expressed in baculovirus infected insect cells
assay   ≥90% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 51 kDa (EED)
  mol wt 86 kDa (EZH2)
  mol wt 87 kDa (SUZ12)
NCBI accession no.   NM_003797 (EED)
  NM_004456 (EZH2)
  NM_015355 (SUZ12)
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... EZH2(2146)



Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Complex of: Human EZH2 (GenBank Accession No. NM_004456), (amino acids 2-end) with N-terminal His tag, MW = 86 kDa. Human EED (GenBank Accession No. NM_003797) (amino acid 2-end) with N-terminal Flag tag, MW = 51 kDa. Human SUZ12, NM_015355, amino acids 2-end, with N-terminal His tag.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 100 μg/ml flag peptide and 10% glycerol.

Price and Availability

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis

Protocols & Articles


Modulators and Proteins for Methylation

DNA methyltransferases are a family of enzymes that catalyze the transfer of a methyl group to DNA. There are five related DNA cytosine-5-methyltransferases (DNMTs) that transfer a methyl group from ...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 7–9
Keywords: Gene expression, Methylations, PAGE

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