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SRP0135 Sigma

G9a human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonym: EHMT2, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, KMT1C, Lysine N-methyltransferase 1C

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Properties

Related Categories Epigenetics, Histone Modifying Enzymes, Methyltransferases, Molecular Biology, Proteins and Peptides for Epigenetic Research More...
recombinant   expressed in E. coli
assay   ≥70% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 74.6 kDa
NCBI accession no.   NM_006709
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... EHMT2(10919)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Human G9a (euchromatic histone-lysine N-methyltransferase 2) (GenBank Accession No. NM_006709) (amino acids 785-1210), with N-terminal GST tag, MW = 74.6 kDa, expressed in an E. coli expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles

Articles

Modulators and Proteins for Methylation

DNA methyltransferases are a family of enzymes that catalyze the transfer of a methyl group to DNA. There are five related DNA cytosine-5-methyltransferases (DNMTs) that transfer a methyl group from ...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 7–9
Keywords: Gene expression, Methylations, PAGE

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