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SRP0145 Sigma

PRMT5/MEP50 Active human

recombinant, expressed in FreeStyle 293-F cells, ≥60% (SDS-PAGE)

Synonym: Arginine methyltransferase 5, HMT1 hnRNP methyltransferase-like 5(HRMT1L5), ICln-binding protein (IBP72) 72 kDa, Jak-binding protein 1 (JBP1), shk1 kinase-binding protein 1 homolog (SKB1)

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Properties

Related Categories Epigenetics, Histone Modifying Enzymes, Methyltransferases, Molecular Biology, Proteins and Peptides for Epigenetic Research More...
recombinant   expressed in FreeStyle 293-F cells
assay   ≥60% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 37.5 kDa (MEP50)
  mol wt 73 kDa (PRMT5)
NCBI accession no.   NM_006109 (PRMT5)
  NM_024102 (MEP50)
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PRMT5(10419)

Description

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 80 μg/ml FLAG® peptide, and 20% glycerol.

Application

Recombinant PRMT5/MEP50 has been used in the detection of arginine methylation in in vitro methyltransferase assay.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Methylosome protein 50 (MEP50) binds to the oncogenic protein arginine methyltransferase 5 (PRMT5) and forms a complex called methylosome. This complex is involved in mono- and symmetric dimethylation of arginine, which is necessary for various biological processes. MEP50 acts as a coactivator for androgen receptor (AR) and estrogen receptor (ER). PRMT5 also methylates histones and other proteins involved in genome organization, transcription, stem cells, primordial germ cells, differentiation, the cell cycle, and spliceosome assembly. The two proteins have been associated with cancer tumorigenesis and are marked as potential oncogenes.

General description

Protein arginine methyltransferase 5 (PRMT5) and WD repeat-containing protein (WDR77) or methylosome protein- 50 (MEP-50) are part of the methylosome. PRMT5 is a type II PRMT enzyme. The gene encoding it is localized to human chromosome 14q11.2.
Complex of human PRMT5 (GenBank Accession No. NM_006109), amino acid 2-end, with N-terminal FLAG®-tag, MW=73 kDa, and human MEP50 (GenBank Accession No. NM_024102), amino acid 2-end, with N-terminal His tag, MW=37.5 kDa, expressed in FreeStyle 293-F cells.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FreeStyle is a trademark of Invitrogen Corp.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Modulators and Proteins for Methylation

DNA methyltransferases are a family of enzymes that catalyze the transfer of a methyl group to DNA. There are five related DNA cytosine-5-methyltransferases (DNMTs) that transfer a methyl group from ...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 7–9
Keywords: Gene expression, Methylations, PAGE

Peer-Reviewed Papers
15

References

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